β1 integrin goat pab Search Results


anti lap tgf β1 goat igg abs  (R&D Systems)
95
R&D Systems anti lap tgf β1 goat igg abs
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Anti Lap Tgf β1 Goat Igg Abs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/Goat+IgG+PE-conjugated+Antibody/pmc02193942-28-25-33
Average 95 stars, based on 1 article reviews
anti lap tgf β1 goat igg abs - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
goat anti γ tubulin  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology goat anti γ tubulin
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Goat Anti γ Tubulin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/TR%CE%B11%2F%CE%B21+Antibody/pmc02223401-49-3-17
Average 94 stars, based on 1 article reviews
goat anti γ tubulin - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
integrin β1 antibody n 20 goat polyclonal antibody  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology integrin β1 antibody n 20 goat polyclonal antibody
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Integrin β1 Antibody N 20 Goat Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/Integrin+%CE%B21+Antibody/pm24955499-35-18-37
Average 96 stars, based on 1 article reviews
integrin β1 antibody n 20 goat polyclonal antibody - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
goat anti human tgf β1  (R&D Systems)
93
R&D Systems goat anti human tgf β1
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Goat Anti Human Tgf β1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/Human+LAP+(TGF-beta+1)+Antibody/pmc04720443-43-0-13
Average 93 stars, based on 1 article reviews
goat anti human tgf β1 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
goat anti rabbit hrp  (Jackson Immuno)
96
Jackson Immuno goat anti rabbit hrp
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Goat Anti Rabbit Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/Goat+Anti-Rabbit+IgG/pmc06122953-176-19-24
Average 96 stars, based on 1 article reviews
goat anti rabbit hrp - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
goat anti mouse β1 integrin antibody  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology goat anti mouse β1 integrin antibody
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Goat Anti Mouse β1 Integrin Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/goat+anti-mouse+IgG-B/pmc03584858-203-14-18
Average 96 stars, based on 1 article reviews
goat anti mouse β1 integrin antibody - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
β1 integrin  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology β1 integrin
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
β1 Integrin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/Integrin+%CE%B21+Lentiviral+Activation+Particles/pmc05503713-3-0-5
Average 93 stars, based on 1 article reviews
β1 integrin - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
tgf β1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology tgf β1
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Tgf β1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/TGF%CE%B21/pm17208206-63-51-56
Average 96 stars, based on 1 article reviews
tgf β1 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
mouse/rat integrin beta 1/cd29 antibody  (Bio-Techne corporation)
94
Bio-Techne corporation mouse/rat integrin beta 1/cd29 antibody
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Mouse/Rat Integrin Beta 1/Cd29 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/Mouse%2FRat+Integrin+beta+1%2FCD29+Antibody/custom%40af2405%4038650985
Average 94 stars, based on 1 article reviews
mouse/rat integrin beta 1/cd29 antibody - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
goat serum  (Proteintech)
96
Proteintech goat serum
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Goat Serum, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/TGF-beta+1+Antibody/pmc10671059-159-12-37
Average 96 stars, based on 1 article reviews
goat serum - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
goat polyclonal anti lamin b1  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology goat polyclonal anti lamin b1
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Goat Polyclonal Anti Lamin B1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/karyopherin+%CE%B21+Antibody/10__1523_slash_jneurosci__0730___17__2017-99-63-69
Average 94 stars, based on 1 article reviews
goat polyclonal anti lamin b1 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
goat anti human lap β1  (R&D Systems)
93
R&D Systems goat anti human lap β1
Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or <t>anti–TGF-β1</t> mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.
Goat Anti Human Lap β1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin+goat+pab/Human+LAP+TGF-beta+1+Biotinylated+Antibody/pmc03889641-137-15-18
Average 93 stars, based on 1 article reviews
goat anti human lap β1 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

Image Search Results


Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or anti–TGF-β1 mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.

Journal: The Journal of Experimental Medicine

Article Title: Phenotype, Localization, and Mechanism of Suppression of CD4 + CD25 + Human Thymocytes

doi: 10.1084/jem.20020110

Figure Lengend Snippet: Cell contact–dependent suppressive activity of CD4 + CD25 + human thymocytes is abrogated by the combined action of anti–CTLA-4 and anti-TGFβ1 mAbs. (A) CD4 + CD25 − thymocytes were stimulated with irradiated T cell–depleted allogeneic PBMNCs in the lower chamber of a transwell plate in the absence or presence of CD4 + CD25 + thymocytes, placed in the same or in the top chamber. On day 5, cells present in the bottom chamber were harvested and their proliferation assessed by measuring 3 [H]TdR uptake. (B) CD4 + CD25 − thymocytes were stimulated with allogeneic irradiated T cell–depleted PBMNCs in the presence of different numbers of autologous purified CD4 + CD25 + thymocytes without (black columns) or with isotype control (IgG1+IgG2a) (gray columns), anti-CTLA-4 (hatched columns), or anti–TGF-β1 mAb (dotted columns), or mixtures of them (white columns). Cell proliferation was measured by assessment of 3 [H]TdR uptake. Mean values (±SD) obtained in four separate experiments are reported.

Article Snippet: The PE-conjugated anti-TNFR2 (22235.311, mouse IgG2a), the purified anti–TGF-β1 (9016.2, mouse IgG1) neutralizing mAb, TGF-βR2 (goat IgG) neutralizing Ab, and biotin-conjugated anti–TGF-β1 (chicken IgY) and anti-LAP (TGF-β1) (goat IgG) Abs were purchased from R&D Systems.

Techniques: Activity Assay, Irradiation, Purification, Control

CTLA-4 and TGF-β1–mediated suppression is due to the inhibition of IL-2R α-chain expression and is overcome by IL-15. (A) CFSE-labeled CD4 + CD25 − thymocytes were stimulated with irradiated allogeneic PB non-T cells in absence or presence of autologous CD4 + CD25 + thymocytes with anti–CTLA-4, anti–TGF-β1, anti–CTLA-4 plus anti–TGF-β1 or isotype-matched (IgG1 plus IgG2a) mAbs, and assessed by flow cytometry for proliferation and surface CD25 expression. One representative of three separate experiments is shown. (B) CD25 (left) and CD69 (right) expression by CD4 + CD25 − thymocytes cultured under the experimental conditions mentioned in (A). CD4 + CD25 − thymocytes without mAb added (black columns), or containing isotype control (gray columns), anti–TGF-β1 (dotted columns), anti–CTLA-4 (hatched columns), or anti–CTLA-4 plus anti–TGF-β1 (white columns) mAbs. Columns represent mean values (±SD) found in three separate experiments. (C) Effect of the addition of exogenous IL-2 or IL-15 on the proliferative response of CD4 + CD25 − thymocytes stimulated with irradiated allogeneic non-T cells in absence or presence of CD4 + CD25 + autologous thymocytes. Columns represent mean values (±SD) obtained in three separate experiments.

Journal: The Journal of Experimental Medicine

Article Title: Phenotype, Localization, and Mechanism of Suppression of CD4 + CD25 + Human Thymocytes

doi: 10.1084/jem.20020110

Figure Lengend Snippet: CTLA-4 and TGF-β1–mediated suppression is due to the inhibition of IL-2R α-chain expression and is overcome by IL-15. (A) CFSE-labeled CD4 + CD25 − thymocytes were stimulated with irradiated allogeneic PB non-T cells in absence or presence of autologous CD4 + CD25 + thymocytes with anti–CTLA-4, anti–TGF-β1, anti–CTLA-4 plus anti–TGF-β1 or isotype-matched (IgG1 plus IgG2a) mAbs, and assessed by flow cytometry for proliferation and surface CD25 expression. One representative of three separate experiments is shown. (B) CD25 (left) and CD69 (right) expression by CD4 + CD25 − thymocytes cultured under the experimental conditions mentioned in (A). CD4 + CD25 − thymocytes without mAb added (black columns), or containing isotype control (gray columns), anti–TGF-β1 (dotted columns), anti–CTLA-4 (hatched columns), or anti–CTLA-4 plus anti–TGF-β1 (white columns) mAbs. Columns represent mean values (±SD) found in three separate experiments. (C) Effect of the addition of exogenous IL-2 or IL-15 on the proliferative response of CD4 + CD25 − thymocytes stimulated with irradiated allogeneic non-T cells in absence or presence of CD4 + CD25 + autologous thymocytes. Columns represent mean values (±SD) obtained in three separate experiments.

Article Snippet: The PE-conjugated anti-TNFR2 (22235.311, mouse IgG2a), the purified anti–TGF-β1 (9016.2, mouse IgG1) neutralizing mAb, TGF-βR2 (goat IgG) neutralizing Ab, and biotin-conjugated anti–TGF-β1 (chicken IgY) and anti-LAP (TGF-β1) (goat IgG) Abs were purchased from R&D Systems.

Techniques: Inhibition, Expressing, Labeling, Irradiation, Flow Cytometry, Cell Culture, Control